A simple graphical method for determining the inhibition constants of mixed, uncompetitive and non-competitive inhibitors.
نویسنده
چکیده
A new method is described for plotting kinetic results for inhibited enzyme-catalysed reactions. It provides a simple way of determining the inhibition constant, K'(i), of an uncompetitive, mixed or non-competitive inhibitor.
منابع مشابه
Relationships between inhibition constants, inhibitor concentrations for 50% inhibition and types of inhibition: new ways of analysing data.
The concentration of an inhibitor that decreases the rate of an enzyme-catalysed reaction by 50%, symbolized i(0.5), is often used in pharmacological studies to characterize inhibitors. It can be estimated from the common inhibition plots used in biochemistry by means of the fact that the extrapolated inhibitor concentration at which the rate becomes infinite is equal to -i(0.5). This method is...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 137 1 شماره
صفحات -
تاریخ انتشار 1974